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Visualisering av Cortex Organisasjon og Dynamics i Mikroorganismer, bruke Total Internal Reflection fluorescensmikroskopi
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Chapters
- 00:05Title
- 01:21Sample Preparation: Preparing Cover Slips
- 02:27Sample Preparation: Preparing Bacillus subtilis Cells
- 03:58Sample Preparation: Preparing Saccharomyces cerevisiae Cells
- 05:03Image Acquisition
- 09:44Results: Visualization of Cortex Organization and Dynamics
- 13:41Conclusion
Total Internal Reflection Fluorescens (TIRF) mikroskopi er en kraftig tilnærming å observere strukturer nær celleoverflaten ved høy kontrast og tidsmessig oppløsning. Vi viser hvordan TIRF kan brukes til å studere protein dynamikk i cortex av celleveggen lukkede bakterie og sopp celler.
Tags
Cortex OrganizationDynamicsMicroorganismsTotal Internal Reflection Fluorescence MicroscopyTIRF MicroscopySpatio-temporal DynamicsFluorescent MoleculesTotal Internal ReflectionCritical AngleEvanescent WaveAxial ResolutionSignal To Noise RatioPhotobleachingWidefield TechniqueScanning-based Confocal SetupsPenetration DepthBacterial CellsFungal CellsCell WallsObservable Structures
