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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
JoVE Journal
Medicine
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JoVE Journal Medicine
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
DOI:

08:45 min

December 28, 2017

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Chapters

  • 00:05Title
  • 01:11Tagmentation and PCR Amplification
  • 03:35PCR Cleanup
  • 05:15Library Normalization
  • 07:19Results: Susceptibility Profiles of Candida glabrata Isolates Correlates with Presence or Absence of Specific Gene Mutations
  • 08:11Conclusion

Summary

Automatic Translation

This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.

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