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Journal / Genetics / 转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件…
JoVE Journal
Genetics

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JoVE Journal Genetics
转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件
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转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件

Article DOI: 10.3791/56752-v • 12:54 min • March 7th, 2018
March 7th, 2018 •
*1, *1, 2, 1,3
1Institute for Diabetes and Obesity (IDO), German Center for Diabetes Research (DZD), Helmholtz Zentrum München, 2Institute for Informatics, Ludwig-Maximilians-Universität München, 3Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Penzberg
* These authors contributed equally

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该协议描述了芯片序列的组合使用, 4 的 su 序列, 总 RNA 序列, 和核糖体分析的细胞系和主细胞。它能够跟踪转录因子绑定、从头转录、RNA 处理、营业额和翻译的变化, 并在激活和/或快速变化的细胞中显示事件的总体过程。

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遗传学 问题 133 4-thiouridine 核糖体分析 芯片序列 rna 序 新转录 RNA 全基因组 rna 处理 剪接 cotranscriptional 剪接 翻译率 更替率 转录因子结合
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