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Journal / Developmental Biology / Strømlinet 3D cerebellare differentiering…
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Developmental Biology

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JoVE Journal Developmental Biology
Strømlinet 3D cerebellare differentiering protokol med valgfri 2D ændring
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Strømlinet 3D cerebellare differentiering protokol med valgfri 2D ændring

Article DOI: 10.3791/56888-v • 10:15 min • December 9th, 2017
December 9th, 2017 •
1, 1,2
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Vi beskriver et forenklet 3D differentiering protokol for hPSCs, bruger defineret medium og reduceret vækstfaktorer, frembringe celle aggregater med tidlige neuroepithelial strukturer og positiv for cerebellare-associerede markører, samt en valgfri 2D modifikation for differentiere celler som en éncellelag til at generere funktionelle neuroner.

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Udviklingsmæssige biologi emnet 130 3D organoid pluripotente stamceller lillehjernen granula celle cortex
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