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Developmental Biology

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JoVE Journal Developmental Biology
Generering af 3D-kugler og 2D luft-væske-grænsefladekulturer af humane inducerede pluripotente stamcelleafledte type 2 alveolære epitelceller
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
Journal (Developmental Biology)
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging (Video) | JoVE
Journal (Developmental Biology)
Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging (Video) | JoVE
 
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Generering af 3D-kugler og 2D luft-væske-grænsefladekulturer af humane inducerede pluripotente stamcelleafledte type 2 alveolære epitelceller

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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Den foreliggende protokol beskriver humant inducerede pluripotente stamcelleafledte type 2 alveolære epitellignende celler (iAT2s). Disse celler kan dyrkes som selvfornyende kugler i 3D-kultur eller tilpasses luft-væske-interface (ALI) kultur.

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Udviklingsbiologi udgave 182
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