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JoVE Journal
Developmental Biology

This content is Open Access.

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
 

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Article doi: 10.3791/56888
December 9th, 2017

Summary December 9th, 2017

We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.

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