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JoVE Journal Cancer Research
Imaging-Ansätze zur Bewertung der toxikologischen oxidativen Stress mit genetisch codierte Fluorogenic Sensoren
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Imaging-Ansätze zur Bewertung der toxikologischen oxidativen Stress mit genetisch codierte Fluorogenic Sensoren

Article DOI: 10.3791/56945-v • 09:33 min • February 7th, 2018
February 7th, 2018 •
1, 2, 2,3
1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

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Dieses Manuskript beschreibt die Verwendung von genetisch codierte Fluorogenic Reporter in einer live Cell Imaging-Anwendung für die Prüfung der Harnblase-induzierten oxidativen Stress. Diese experimentelle Ansatz bietet unvergleichliche räumlich-zeitliche Auflösung, Sensitivität und Spezifität vieler die Unzulänglichkeiten der konventionellen Methoden zur Erkennung von toxikologischen oxidativen Stress zu vermeiden.

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Cancer Research Ausgabe 132 oxidativer Stress Peroxid Toxikologie Fluoreszenz Live-Cell imaging Glutathion Wasserstoff
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