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Immunology and Infection

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JoVE Journal Immunology and Infection
En Protein Microarray analys för serologisk bestämning av Antigen-specifika antikroppen Svaren följande Clostridium difficile infektion
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En Protein Microarray analys för serologisk bestämning av Antigen-specifika antikroppen Svaren följande Clostridium difficile infektion

Article DOI: 10.3791/57399-v • 09:12 min • June 15th, 2018
June 15th, 2018 •
1,2, 1,2, 3
1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

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Denna artikel beskriver en enkel protein microarray metod för profilering humorala immunsvar mot en 7-plex panel av mycket renad Clostridium difficile antigener i humant serum. Protokollet kan förlängas för bestämning av specifika antikroppen svaren i förberedelserna av polyklonala intravenöst immunglobulin.

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Immunologi och infektion fråga 136 Protein microarray antikropp Clostridium difficile intravenös immunglobulin
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