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Immunology and Infection
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JoVE Journal Immunology and Infection
Kolorektal cancer cellytan Proteinprofilering Använda en antikropp microarray och fluorescens Multiplexing
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Kolorektal cancer cellytan Proteinprofilering Använda en antikropp microarray och fluorescens Multiplexing

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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Vi beskrev ett förfarande för uppdelning av kolorektal cancer (CRC) för att producera livskraftiga enskilda celler, som sedan fångas på skräddarsydda antikroppar microarrays erkänna ytantigener (DotScan CRC microarray). Sub-populationer av celler bundna till microarray kan profileras med fluorescens multiplexering använda monoklonala antikroppar märkta med fluorescerande färger.

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Immunologi 55 kolorektal cancer leukocyter antikropp microarray multiplexering fluorescens CD-antigener
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