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Immunology and Infection

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JoVE Journal Immunology and Infection
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Un'analisi di Microarray della proteina per determinazione sierologica dell'infezione di Clostridium difficile anticorpo seguenti risposte antigene-specifiche

Article DOI: 10.3791/57399-v • 09:12 min • June 15th, 2018
June 15th, 2018 •
1,2, 1,2, 3
1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

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Questo articolo viene descritto un metodo di microarray della proteina semplice per profilatura risposte immunitarie umorali a un panel 7-plex di altamente purificata antigeni di Clostridium difficile in sieri umani. Il protocollo può essere esteso per la determinazione della risposta di anticorpo specifico nelle preparazioni a base di immunoglobuline policlonali.

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Immunologia e infezione problema 136 proteina microarray anticorpo l'immunoglobulina endovenosa Clostridium difficile,
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