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In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
JoVE Journal
Environment
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JoVE Journal Environment
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
DOI:

07:24 min

August 31, 2018

Chapters

  • 00:04Title
  • 00:52Removal of Paraffin
  • 01:44Pretreatment of Slides for Preparation of RNA Probe Hybridization
  • 03:03Hybridization of the RNA Probe
  • 04:07Visualization of the RNA Probe
  • 06:18Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
  • 07:07Conclusion

Summary

Automatic Translation

The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.

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