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Chapters
- 00:04Title
- 00:52Removal of Paraffin
- 01:44Pretreatment of Slides for Preparation of RNA Probe Hybridization
- 03:03Hybridization of the RNA Probe
- 04:07Visualization of the RNA Probe
- 06:18Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
- 07:07Conclusion
The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.
