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JoVE Journal Genetics
High-throughput DNA-extractie en de genotypering van 3dpf Zebrafish larven door Fin knippen
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High-throughput DNA-extractie en de genotypering van 3dpf Zebrafish larven door Fin knippen

Article DOI: 10.3791/58024-v • 10:12 min • June 29th, 2018
June 29th, 2018 •
1,2, 1, 1,2, 1,2, 1,2, 1, 1, 2, 2, 1, 1,2
1Children's Hospital of Eastern Ontario Research Institute, 2Department of Biology, University of Ottawa

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Zebravis als betrouwbare genetische modelorganismen in biomedisch onderzoek, vooral met de komst van gen-bewerken technologieën zijn gebruikt. Wanneer larvale fenotypen verwachting, kan DNA-extractie en genotype identificatie worden uitdagende. Hier beschrijven we een efficiënte genotypering procedure voor zebrafish larven, door de staart knippen, zo spoedig 72 uur na bevruchting.

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Genetica kwestie 136 zebravis Danio rerio genotypering larvale fin knippen DNA-extractie
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