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Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
DOI:

08:58 min

November 19, 2018

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Chapters

  • 00:04Title
  • 01:173D Quantitative Phase Imaging
  • 04:34Quantitative Morphological and Biochemical Feature Extraction and Supervised Learning and Identification
  • 06:42Results: Representative Label-Free Lymphocyte Subtype 3D Phase Imaging Identification
  • 07:56Conclusion

Summary

Automatic Translation

We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell types.

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