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Journal / Cancer Research / Optrævling nøglespillere humorale immunitet:…
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Optrævling nøglespillere humorale immunitet: avancerede og optimeret lymfocyt Isolation protokol fra Murine Peyer's Patches

Article DOI: 10.3791/58490-v • 08:25 min • November 21st, 2018
November 21st, 2018 •
1,2, 1,2, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School

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I denne undersøgelse præsenterer vi en roman og effektiv protokol til isolering af lymfocytter fra Peyerske plaques (PPs), som senere kan bruges til i vivo og in vitro- funktionelle assays samt flow cytometric undersøgelser af follikulært T hjælperen og germinal center B celler.

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Kræftforskning sag 141 Peyerske plaques follikulært T helper celler germinal center B-celle lymfocyt isolation væv forberedelse lymfocyt delmængder lymfoide organer collagenase
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