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Journal / Developmental Biology / RNA-ha basato riprogrammazione dei fibroblasti…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
RNA-ha basato riprogrammazione dei fibroblasti primari umani in cellule staminali pluripotenti indotte
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RNA-ha basato riprogrammazione dei fibroblasti primari umani in cellule staminali pluripotenti indotte

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Qui descriviamo un metodo clinicamente rilevante, ad alta efficienza e privo di alimentatore per riprogrammare i fibroblasti primari umani in cellule staminali pluripotenti indotte utilizzando modificate mRNA che codificano per fattori di riprogrammazione e maturo microRNA-367/302 imita. Sono inoltre inclusi metodi per valutare l'efficienza di riprogrammazione, espandere le colonie clonali iPSC e confermare l'espressione del marcatore pluripotenza TRA-1-60.

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Problema 141 biologia dello sviluppo fibroblasti cellule staminali pluripotenti indotte (iPSCs) riprogrammazione umano modificate mRNAs clinicamente rilevanti i microRNA (Mirna)
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