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En Rhodopsin Transport Assay af High-indhold Imaging analyse
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En Rhodopsin Transport Assay af High-indhold Imaging analyse

Article DOI: 10.3791/58703-v • 12:11 min • January 16th, 2019
January 16th, 2019 •
1, 1, 1,2
1Department of Ophthalmology, University of Pittsburgh, 2McGowan Institute for Regenerative Medicine, University of Pittsburgh

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Her, beskrevet vi en høj-indhold billedbehandling metode for at kvantificere transport af rhodopsin mutanter tilknyttet retinitis pigmentosa. En multiple-bølgelængde scoring analyse blev brugt til at kvantificere rhodopsin protein på cellens overflade eller i hele cellen.

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Kemi spørgsmålet 143 High-indhold imaging fejlfoldede protein rhodopsin retinitis pigmentosa farmakologiske anstandsdame immunfarvning
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