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Journal / Biochemistry / 荧光显微镜竞争滴定法测定转录机-dna…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
荧光显微镜竞争滴定法测定转录机-dna 结合感的高灵敏度
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Journal (Biochemistry)
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Journal (Biochemistry)
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
 
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荧光显微镜竞争滴定法测定转录机-dna 结合感的高灵敏度

Article DOI: 10.3791/58763-v • 06:38 min • February 7th, 2019
February 7th, 2019 •
1, 1, 1, 1, 1
1Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität München

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在这里, 我们提出了一种新的方法来确定结合亲和力的平衡和解决具有高灵敏度的大尺度。这改进了转录因子-dna 结合的定量分析。该方法基于控制下的输送系统中的自动荧光各向异性测量。

Tags

生物化学 第144期 转录因子 蛋白质-dna 相互作用 结合特异性 结合亲和力 荧光各向异性 分割网络
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