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Isolamento, purificazione e differenziazione dei precursori dell'osteoclast dal midollo osseo del ratto
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Isolamento, purificazione e differenziazione dei precursori dell'osteoclast dal midollo osseo del ratto

Article DOI: 10.3791/58895-v • 11:11 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 3, 1,2,4
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

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Gli osteoclasti sono polykaryons di macrofage specifici del tessuto derivato dal lignaggio dei monociti-macrofage delle cellule staminali ematopoietiche. Questo protocollo descrive come isolare le cellule del midollo osseo in modo da ottenere grandi quantità di osteoclasti riducendo il rischio di incidenti riscontrati nei metodi tradizionali.

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Biologia problema 147 osteoclast osteoporosi recettore attivatore per il Ligato di fattore-κB nucleare (RANKL) rimodellamento osseo biologia cellulare isolamento riassorbimento osseo
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