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JoVE Journal Biology
Isolamento e differenziazione dei preadipociti bianchi e marroni primari dai topi appena nati
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Isolamento e differenziazione dei preadipociti bianchi e marroni primari dai topi appena nati

Article DOI: 10.3791/62005-v • 09:00 min • January 25th, 2021
January 25th, 2021 •
1,2, 1, 1
1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA, 2Department of Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

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Questo rapporto descrive un protocollo per l'isolamento simultaneo dei preadipociti primari marroni e bianchi dai topi appena nati. Le cellule isolate possono essere coltivate in coltura e indotte a differenziarsi in adipociti bianchi e marroni completamente maturi. Il metodo consente la caratterizzazione genetica, molecolare e funzionale delle cellule adipose primarie in coltura.

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Questo mese in JoVE Numero 167 Adipociti bianchi adipociti marroni topo cultura primaria adipogenesi obesità diabete malattie metaboliche
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