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JoVE Journal Cancer Research
Evaluatie van Biomarkers in Glioma door Immunohistochemistry op 3D Glioma paraffine-ingebedde Neurosphere culturen
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Evaluatie van Biomarkers in Glioma door Immunohistochemistry op 3D Glioma paraffine-ingebedde Neurosphere culturen

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurospheres gegroeid als 3D culturen vormen een krachtig hulpmiddel om te studeren glioma biologie. Hier presenteren we een protocol voor het uitvoeren van immunohistochemistry met behoud van de 3D structuur van glioma neurospheres via paraffine insluiten. Deze methode kan de karakterisatie van glioma neurosphere eigenschappen zoals stemness en Neurale differentiatie.

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Kankeronderzoek kwestie 143 genetisch gemanipuleerde glioma neurospheres 3D-culturen immunokleuring biomarkers paraffine insluiten
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