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Valutazione di biomarcatori in Glioma mediante immunoistochimica su paraffina 3D Glioma Neurosphere culture
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Valutazione di biomarcatori in Glioma mediante immunoistochimica su paraffina 3D Glioma Neurosphere culture

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurosfere coltivate come 3D culture costituiscono un potente strumento per studiare la biologia di glioma. Qui presentiamo un protocollo per eseguire esame immuno-istochimico pur mantenendo la struttura 3D di glioma neurosfere mediante inclusione in paraffina. Questo metodo consente la caratterizzazione delle proprietà neurosphere glioma ad esempio di staminalità e del differenziamento neurale.

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Ricerca sul cancro numero 143 geneticamente glioma neurosfere 3D-culture immunostaining biomarcatori inclusione in paraffina
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