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JoVE Journal Cancer Research
Evaluering av Biomarkers i Glioma av immunhistokjemi på parafin-Embedded 3D Glioma Neurosphere kulturer
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Evaluering av Biomarkers i Glioma av immunhistokjemi på parafin-Embedded 3D Glioma Neurosphere kulturer

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurospheres dyrket som 3D kulturer utgjør et kraftig verktøy for å studere glioma biologi. Her presenterer vi en protokoll for å utføre immunohistochemistry samtidig 3D oppbygning glioma neurospheres gjennom parafininnstøper. Denne metoden kan karakterisering av glioma neurosphere egenskaper som stemness og nevrale differensiering.

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Kreftforskning problemet 143 genmodifisert glioma neurospheres 3D-kulturer immunostai- biomarkers parafin innebygging
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