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Journal / Neuroscience / En rustfritt protokoll for høykvalitets RNA…
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Neuroscience

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JoVE Journal Neuroscience
En rustfritt protokoll for høykvalitets RNA isolasjon fra Laser fange Microdissected Purkinje celler i menneskelig obduksjon lillehjernen
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En rustfritt protokoll for høykvalitets RNA isolasjon fra Laser fange Microdissected Purkinje celler i menneskelig obduksjon lillehjernen

Article DOI: 10.3791/58953-v • 09:04 min • January 17th, 2019
January 17th, 2019 •
1, 2,3,4, 1
1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

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Denne protokollen bruker en flekk-fri tilnærming til å visualisere og isolere Purkinje celler i fersk-frosne vev fra menneskelige obduksjon lillehjernen via laser fange microdissection. Formålet med denne protokollen er å generere tilstrekkelig mengder av høy kvalitet for RNA-sekvensering.

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Nevrovitenskap problemet 143 Purkinje celler lillehjernen laser fange microdissection RNA RNAseq viktig tremor
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