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Un protocolo de acero inoxidable para el aislamiento de ARN de alta calidad del Laser capturar las células Microdissected de Purkinje en el cerebelo humano Post-Mortem
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1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University
Chapters
- 00:04Title
- 00:33Prepare Cryostat for Tissue Sectioning
- 01:51Sectioning Tissue
- 03:50Fixing Tissue/Stain-less Visualization
- 04:47Laser Capture Microdissection of Purkinje Cells
- 06:09Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
- 07:59Conclusion
Este protocolo utiliza un enfoque libre de manchas para visualizar y aislar a las células de Purkinje en tejido fresco congelado del cerebelo humano post-mortem mediante microdisección de captura láser. El propósito de este protocolo es generar cantidades suficientes de ARN de alta calidad para secuenciación del RNA.
