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Journal / Biochemistry / Übersetzen von Ribososomen-Affinitätsreinigung…
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Biochemistry
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JoVE Journal Biochemistry
Übersetzen von Ribososomen-Affinitätsreinigung (TRAP) zur RNA-Isolierung von Endothelzellen In vivo
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Übersetzen von Ribososomen-Affinitätsreinigung (TRAP) zur RNA-Isolierung von Endothelzellen In vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Wir präsentieren einen Ansatz zur Reinigung ribossomengebundener mRNA aus vaskulären Endothelzellen (ECs) direkt im Gehirn, der Lunge und im Herzgewebe der Maus über das EC-spezifische genetische Tag des verbesserten grünen Fluoreszenzproteins (EGFP) in Ribosomen in Kombination mit RNA-Reinigung .

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Biochemie Ausgabe 147 Angiogenese arteriolare Differenzierung vaskuläre Endothelzellen Übersetzen ribososome Affinitätsreinigung RNA-Extraktion Echtzeit qPCR
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