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Journal / Immunology and Infection / Transfección altamente eficiente de macrófagos…
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JoVE Journal Immunology and Infection
Transfección altamente eficiente de macrófagos primarios con ARNm transcrito in Vitro
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Transfección altamente eficiente de macrófagos primarios con ARNm transcrito in Vitro

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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Los macrófagos, especialmente los macrófagos primarios, son difíciles de transfectar, ya que se especializan en la detección de moléculas de origen no propio. Describimos un protocolo que permite la transfección altamente eficiente de macrófagos primarios con ARNm generados a partir de plantillas de ADN como plásmidos.

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Inmunología e infección número 153 macrófagos primarios macrófagos peritoneales macrófagos derivados de la médula ósea células inmunitarias transcripción in vitro ARNm transfección
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