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Journal
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Immunology and Infection
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具有体外转录mRNA的初级巨噬细胞的高效转染
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
Please note that all translations are automatically generated.
Click here for the English version.
具有体外转录mRNA的初级巨噬细胞的高效转染
DOI:
10.3791/60143-v
•
06:46 min
•
November 09, 2019
•
Marc Herb
,
Alina Farid
,
Alexander Gluschko
,
Martin Krönke
2,3,4
,
Michael Schramm
1
Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne
,
University of Cologne
,
2
Center for Molecular Medicine Cologne
,
3
Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD)
,
4
German Center for Infection Research (DZIF)
Chapters
00:04
Title
00:43
mRNA Generation and Purification
02:59
Transfection of Macrophages with mRNA
04:27
Results: Highly Efficient Transfection of Peritoneal Macrophages and Bone Marrow-Derived Macrophages with in vitro Transcribed mRNA
06:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
巨噬细胞,特别是原发性巨噬细胞,由于它们专门检测非自源分子,因此很难转染。我们描述了一种协议,它允许使用从DNA模板(如质粒)生成的mRNA对原性巨噬细胞进行高效的转染。
Tags
Transfection
Primary Macrophages
In Vitro Transcribed MRNA
RNA Transcription
PolyA Tailing
Dephosphorylation
MRNA Purification
Denaturing Agarose Gel
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