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具有体外转录mRNA的初级巨噬细胞的高效转染
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
DOI:

06:46 min

November 09, 2019

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Chapters

  • 00:04Title
  • 00:43mRNA Generation and Purification
  • 02:59Transfection of Macrophages with mRNA
  • 04:27Results: Highly Efficient Transfection of Peritoneal Macrophages and Bone Marrow-Derived Macrophages with in vitro Transcribed mRNA
  • 06:14Conclusion

Summary

Automatic Translation

巨噬细胞,特别是原发性巨噬细胞,由于它们专门检测非自源分子,因此很难转染。我们描述了一种协议,它允许使用从DNA模板(如质粒)生成的mRNA对原性巨噬细胞进行高效的转染。

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