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En Ornesen Endothelial organ kultur model ved hjælp af Split cornea-knapper

Article DOI: 10.3791/60171-v • 08:36 min • October 6th, 2019
October 6th, 2019 •
1, 2, 1, 1, 1
1Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), 2University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen

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Her præsenteres en trin-for-trin-protokol til tilberedning og dyrkning af Porcin split cornea-knapper. Da denne organo-typisk dyrkede organ kulturmodel viser celledødelighed inden for 15 dage, sammenlignelige med human donor hornhinder, det repræsenterer den første model, der tillader langvarig dyrkning af ikke-humane hornhinder uden at tilføje giftige dextran.

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Medicin organ kulturmodel forskningsmodel hornhinde endothelium cornea endothelium cornea endotheliale celler endotelceller split hornhinde knap grise øjne svin
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