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JoVE Journal Biochemistry
Påvisning af totalreaktive iltarter i klæbende celler med 2',7'-Dichlordihydrofluorescein Diacetate Staining
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Påvisning af totalreaktive iltarter i klæbende celler med 2',7'-Dichlordihydrofluorescein Diacetate Staining

Article DOI: 10.3791/60682-v • 04:53 min • June 23rd, 2020
June 23rd, 2020 •
1, 1
1Department of Biochemistry and Molecular Biology, University of New Mexico

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Her præsenterer vi en protokol til påvisning af total cellulære reaktive iltarter (ROS) ved hjælp af 2',7'-dichlorodihydrofluorescein diacetat (DCFH-DA). Denne metode kan visualisere cellulær ROS lokalisering i klæbende celler med en fluorescens mikroskop og kvantificere ROS intensitet med en fluorescens plade læser. Denne protokol er enkel, effektiv og omkostningseffektiv.

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Biokemi oxidativt stress 2',7'-Dichlordihydrofluorescein diacetate reaktive ilt arter klæbende celle fluorescens mikroskop fluorescens plade læser
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