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JoVE Journal Developmental Biology
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
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Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Article DOI: 10.3791/60936-v • 07:52 min • May 23rd, 2020
May 23rd, 2020 •
1, 1
1Max Planck Institute of Molecular Plant Physiology

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Here we describe a protocol for live cell imaging of the cortical microtubule cytoskeleton at the shoot apical meristem and monitoring its response to changes in physical forces.

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Live Cell Imaging Microtubule Cytoskeleton Micromechanical Manipulation Arabidopsis Shoot Apical Meristem Plants Structural Components Mechanical Forces Genotypes Conditions Arabidopsis Seeds Green Fluorescent Protein Germination Pots Growth Space Temperature Short Day Conditions Long Day Conditions Inflorescence Centimeters Long Shoot Apical Meristem Dissection Forceps Flowers Peduncles Dissecting Dish
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