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Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

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08:52 min

December 22, 2020

DOI:

08:52 min
December 22, 2020

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Summary

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This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.

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