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JoVE Journal
Developmental Biology

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Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging
 

Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

Article DOI: 10.3791/61954-v
December 22nd, 2020

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Summary December 22nd, 2020

This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.

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