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Pressure-Polieren Pipetten für verbesserte Patch-Clamp Recording
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Pressure-Polieren Pipetten für verbesserte Patch-Clamp Recording

Article DOI: 10.3791/964-v • 05:13 min • October 22nd, 2008
October 22nd, 2008 •
1, 1, 1
1Department of Molecular and Cellular Physiology, Stanford University School of Medicine

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Dies ist eine Anleitung zum Ändern der Form von Glas-Mikropipetten. Insbesondere durch Anwendung von Wärme und Luftdruck der Verjüngung ist ohne Erhöhung des Bahnöffnung verbreitert, was zu niedrigeren Pipette Widerstand. Dies ist entscheidend für rauscharme Aufnahmen von kleinen Zellen zu erhalten, sondern ist in vielen Anwendungen.

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Grundlegende Protokolle Elektrophysiologie Patch-Clamp- Voltage-Clamp Biophysik Gigaseal Ionenkanäle
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