In JoVE (1)
Other Publications (8)
- Acta Pharmaceutica (Zagreb, Croatia)
- Acta Crystallographica. Section E, Structure Reports Online
- Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
- Angewandte Chemie (International Ed. in English)
- Bioorganic & Medicinal Chemistry
- Scientific Reports
- Molecules (Basel, Switzerland)
- Zeitschrift Fur Naturforschung. C, Journal of Biosciences
Articles by Ahmed Fouda in JoVE
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules Ahmed Fouda1, Mahasti Tahsini2, Fatemeh Khodayarian2, Fatimah Al-nafisah2, Moutih Rafei1,2,3 1Department of Microbiology, Infectiology and Immunology, Université de Montréal, 2Department of Pharmacology and Physiology, Université de Montréal, 3Molecular Biology Program, Université de Montréal We present in the current study a novel fluorescence-based assay using lymphocytes derived from a transgenic mouse. This assay is suitable for high-throughput screening (HTS) of small molecules endowed with the capacity of either inhibiting or promoting lymphocyte activation.
Other articles by Ahmed Fouda on PubMed
Synthesis of Some New Annulated Pyrazolo-pyrido (or Pyrano) Pyrimidine, Pyrazolopyridine and Pyranopyrazole Derivatives Acta Pharmaceutica (Zagreb, Croatia). Jun, 2004 | Pubmed ID: 15274757 The bifunctional pyrazolopyridine (2) and pyrano-pyrazole (3) derivatives were prepared by the reaction of 2-(2,4-dinitrophenyl)-5-methyl-2,4-dihydro-3H-pyrazol-3-one (1) with p-methoxybenzaldehyde, malononitrile in the presence of ammonium acetate or piperidine, respectively. Compound 2 was used as the key intermediate to prepare the pyrazolo-pyrido-pyrimidine derivatives through its reaction with formic acid, formamide-formic acid-DMF, ammonium thiocyanate or reaction with triethyl orthoformate followed by cyclization with hydrazine hydrate. Reaction of 3 with triethyl orthoformate followed by cyclization with hydrazine hydrate gave the pyrazolo-pyrano-pyrimidine derivative 11. Reaction of ethyl-3-oxo-2-[2-phenyl-diazenyl]butanoate and ethyl 2-[2-(4-chlorophenyl)diazenyl]-3-oxobutanoate with 1 to give the pyrazolone derivatives 13a and 13b, was also considered.
Ethyl 2-amino-4-(4-bromo-phen-yl)-6-meth-oxy-4H-benzo[h]chromene-3-carboxyl-ate Acta Crystallographica. Section E, Structure Reports Online. Mar, 2013 | Pubmed ID: 23476606 In the title compound, C23H20BrNO4, the pyran ring has a flattened boat conformation with the O and methine C atoms lying to one side of the plane [0.160 (5) and 0.256 (6) Å, respectively] defined by the remaining atoms. Nevertheless, the 4H-benzo[h]chromene ring system approximates a plane (r.m.s. deviation = 0.116 Å) with the bromo-benzene ring almost perpendicular [dihedral angle = 83.27 (16)°] and the ester group coplanar [C-C-C-O = 3.4 (5)°]; the meth-oxy substituent is also coplanar [C-O-C-C = 174.5 (3)°]. In addition to an intra-molecular N-H⋯O(ester carbon-yl) hydrogen bond, the ester carbonyl O atom also forms an inter-molecular N-H⋯O hydrogen bond with the second amine H atom, generating a zigzag supra-molecular chain along the c axis in the crystal packing. The chains are linked into layers in the bc plane by N-H⋯Br hydrogen bonds, and these layers are consolidated into a three-dimensional architecture by C-H⋯π inter-actions.
Synthesis, Characterization and Density Functional Theory Investigations of the Electronic, Photophysical and Charge Transfer Properties of Donor-bridge-acceptor Triaminopyrazolo[1,5-a]pyrimidine Dyes Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy. Jul, 2013 | Pubmed ID: 23648968 We have synthesized multifunctional dyes 3-(4-methyl-phenylazo)-6-(4-nitro-phenylazo)-2,5,7-triaminopyrazolo[1,5-a]pyrimidine (4a) and 3-(4-methyl-phenylazo)-6-(4-acetyl-phenylazo)-2,5,7-triaminopyrazolo[1,5-a]pyrimidine (4b), then characterized by IR, (1)H NMR and (13)C NMR techniques. The ground state geometries have been computed by using density functional theory at B3LYP/6-31G(*) level of theory. The absorption spectra have been calculated by using time dependent density functional theory with and without solvent. The highest occupied molecular orbitals (HOMOs) and lowest unoccupied molecular orbitals (LUMOs) are delocalized and localized on throughout the backbone, respectively. Solvent also play important role towards elevating the dipole moment. Significant red shift in absorption wavelengths have been observed in methanol compared to without solvent. We discussed the electron injection, electronic coupling constant and light harvesting efficiency.
A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation Angewandte Chemie (International Ed. in English). Aug, 2015 | Pubmed ID: 26119262 ATP analogues have been powerful compounds for the study of kinase-catalyzed phosphorylation. However, the cell impermeability of ATP analogues has largely limited their use to in vitro lysate-based experiments. Herein, we report the first cell-permeable ATP analogue, ATP-polyamine-biotin (APB). APB is shown to promote biotin labeling of kinase substrates in live cells and has future applications in phosphoprotein purification and analysis. More generally, these studies provide a foundation for the development of additional cell-permeable ATP analogues for cell-signaling research.
The Generality of Kinase-catalyzed Biotinylation Bioorganic & Medicinal Chemistry. Jan, 2016 | Pubmed ID: 26672511 Kinase-catalyzed protein phosphorylation is involved in a wide variety of cellular events. Development of methods to monitor phosphoproteins in normal and diseased states is critical to fully characterize cell signaling. Towards phosphoprotein analysis tools, our lab reported kinase-catalyzed labeling where γ-phosphate modified ATP analogs are utilized by kinases to label peptides or protein substrates with a functional tag. In particular, the ATP-biotin analog was developed for kinase-catalyzed biotinylation. However, kinase-catalyzed labeling has been tested rigorously with only a few kinases, preventing use of ATP-biotin as a general tool. Here, biotinylation experiments, gel or HPLC-based quantification, and kinetic measurements indicated that twenty-five kinases throughout the kinome tree accepted ATP-biotin as a cosubstrate. With this rigorous characterization of ATP-biotin compatibility, kinase-catalyzed labeling is now immediately useful for studying phosphoproteins and characterizing the role of phosphorylation in various biological events.
Proinflammatory Isoforms of IL-32 As Novel and Robust Biomarkers for Control Failure in HIV-infected Slow Progressors Scientific Reports. Mar, 2016 | Pubmed ID: 26978598 HIV-infected slow progressors (SP) represent a heterogeneous group of subjects who spontaneously control HIV infection without treatment for several years while showing moderate signs of disease progression. Under conditions that remain poorly understood, a subgroup of these subjects experience failure of spontaneous immunological and virological control. Here we determined the frequency of SP subjects who showed loss of HIV control within our Canadian Cohort of HIV(+) Slow Progressors and identified the proinflammatory cytokine IL-32 as a robust biomarker for control failure. Plasmatic levels of the proinflammatory isoforms of IL-32 (mainly β and γ) at earlier clinic visits positively correlated with the decline of CD4 T-cell counts, increased viral load, lower CD4/CD8 ratio and levels of inflammatory markers (sCD14 and IL-6) at later clinic visits. We present here a proof-of-concept for the use of IL-32 as a predictive biomarker for disease progression in SP subjects and identify IL-32 as a potential therapeutic target.
Structural Characterization and Antimicrobial Activities of 7H-Benzo[h]chromeno[2,3-d]pyrimidine and 14H-Benzo[h]chromeno[3,2-e][1,2,4]triazolo[1,5-c] Pyrimidine Derivatives Molecules (Basel, Switzerland). Nov, 2016 | Pubmed ID: 27809292 Three new series of chromene molecules have been synthesized in order to explore their antimicrobial activity. The series encompass 2-substituted 14-(4-halophenyl)-12-methoxy-14H-benzo[h]chromeno[3,2-e][1,2,4]-triazolo[1,5-c]pyrimidines 7a-o, 9-benzylideneamino-7-(4-halo-phenyl)-5-methoxy-8-imino-7H-benzo-[h]chromeno[2,3-d]pyrimidines 8a-b and 3-ethoxycarbonyl-14-(4-halophenyl)-12-methoxy-14H-benzo-[h]chromeno[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-2-one derivatives 12a-b. The structure of these novel compounds were confirmed using IR, ¹H- and (13)C-NMR as well as MS spectroscopy. The new compounds were evaluated in vitro for their antimicrobial activity and it was demonstrated that 7H-benzochromenopyrimidine and derivatives of 14H-benzochromenotriazolopyrimidine exhibited the most promising antibacterial activities compared to the reference antimicrobial agents. The structure activity relationship (SAR) studies of the target compounds agreed with the in vitro essays and confirmed higher potent antimicrobial activity against some of the tested microorganisms.
Synthesis, In-vitro Cytotoxicity of 1H-benzo[f]chromene Derivatives and Structure-activity Relationships of the 1-aryl Group and 9-position Zeitschrift Fur Naturforschung. C, Journal of Biosciences. Nov, 2016 | Pubmed ID: 27831925 A series of 1H-benzo[f]chromene-2-carbonitriles was synthesized and evaluated for their cytotoxic activities against MCF-7, HCT-116, and HepG-2 cancer cells. The SAR studies reported that the substitution in the phenyl ring at 1-position of 1H-benzo[f]chromene nucleus with the specific group, H atom, or methoxy group at 9-position increases the ability of the molecule against the different cell lines.