Andreas Bechthold Department of Pharmaceutical Biology and Biotechnology Albert-Ludwigs-Universität Freiburg, Germany Biography Publications Institution JoVE Articles Andreas Bechthold has not added a biography. If you are Andreas Bechthold and would like to personalize this page please email our Author Liaison for assistance. Publications Selection of an Efficient Promoter and Its Application in Toyocamycin Production Improvement in Streptomyces Diastatochromogenes 1628 World Journal of Microbiology & Biotechnology. Feb, 2017 | Pubmed ID: 28058639 The Draft Genome Sequence of Actinokineospora Bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units Molecules (Basel, Switzerland). Nov, 2016 | Pubmed ID: 27886115 Thailandins A and B, New Polyene Macrolactone Compounds Isolated from Actinokineospora Bangkokensis Strain 44EHW(T), Possessing Antifungal Activity Against Anthracnose Fungi and Pathogenic Yeasts Journal of Agricultural and Food Chemistry. Jun, 2016 | Pubmed ID: 27267862 An Influence of the Copy Number of Biosynthetic Gene Clusters on the Production Level of Antibiotics in a Heterologous Host Journal of Biotechnology. Aug, 2016 | Pubmed ID: 27264245 Characterization of Representative RpoB Gene Mutations Leading to a Significant Change in Toyocamycin Production of Streptomyces Diastatochromogenes 1628 Journal of Industrial Microbiology & Biotechnology. Apr, 2016 | Pubmed ID: 26790416 StreptomeDB 2.0--an Extended Resource of Natural Products Produced by Streptomycetes Nucleic Acids Research. Jan, 2016 | Pubmed ID: 26615197 New Simocyclinones: Surprising Evolutionary and Biosynthetic Insights ACS Chemical Biology. Jan, 2016 | Pubmed ID: 26566170 Bacillus Cereus Efflux Protein BC3310 - a Multidrug Transporter of the Unknown Major Facilitator Family, UMF-2 Frontiers in Microbiology. 2015 | Pubmed ID: 26528249 Biosynthesis of the Fluorinated Natural Product Nucleocidin in Streptomyces Calvus Is Dependent on the BldA-Specified Leu-tRNA(UUA) Molecule Chembiochem : a European Journal of Chemical Biology. Nov, 2015 | Pubmed ID: 26374477 Minimum Information About a Biosynthetic Gene Cluster Nature Chemical Biology. Sep, 2015 | Pubmed ID: 26284661 Changing Biosynthetic Profiles by Expressing BldA in Streptomyces Strains Chembiochem : a European Journal of Chemical Biology. Oct, 2015 | Pubmed ID: 26255983 The Gene BldA, a Regulator of Morphological Differentiation and Antibiotic Production in Streptomyces Archiv Der Pharmazie. Jul, 2015 | Pubmed ID: 25917027 Functional Characterization of Different ORFs Including Luciferase-like Monooxygenase Genes from the Mensacarcin Gene Cluster Chembiochem : a European Journal of Chemical Biology. May, 2015 | Pubmed ID: 25907804 Structural Characterization of O- and C-glycosylating Variants of the Landomycin Glycosyltransferase LanGT2 Angewandte Chemie (International Ed. in English). Feb, 2015 | Pubmed ID: 25581707 Complete Genome Sequence of Producer of the Glycopeptide Antibiotic Aculeximycin Kutzneria Albida DSM 43870T, a Representative of Minor Genus of Pseudonocardiaceae BMC Genomics. Oct, 2014 | Pubmed ID: 25301375 Insights into the Bioactivity of Mensacarcin and Epoxide Formation by MsnO8 Chembiochem : a European Journal of Chemical Biology. Mar, 2014 | Pubmed ID: 24554499 Overexpression of Ribosome Recycling Factor is Responsible for Improvement of Nucleotide Antibiotic-toyocamycin in Streptomyces Diastatochromogenes 1628 Applied Microbiology and Biotechnology. Jun, 2014 | Pubmed ID: 24509772 Development of Intergeneric Conjugal Gene Transfer System in Streptomyces Diastatochromogenes 1628 and Its Application for Improvement of Toyocamycin Production Current Microbiology. Feb, 2014 | Pubmed ID: 24057064 Pleiotropic Regulatory Genes BldA, AdpA and AbsB Are Implicated in Production of Phosphoglycolipid Antibiotic Moenomycin Open Biology. Oct, 2013 | Pubmed ID: 24153004 A Cryptic Polyene Biosynthetic Gene Cluster in Streptomyces Calvus is Expressed Upon Complementation with a Functional BldA Gene Chemistry & Biology. Oct, 2013 | Pubmed ID: 24120331 Tracking Down Biotransformation to the Genetic Level: Identification of a Highly Flexible Glycosyltransferase from Saccharothrix Espanaensis Applied and Environmental Microbiology. Sep, 2013 | Pubmed ID: 23793643 A Transposon-based Strategy to Identify the Regulatory Gene Network Responsible for Landomycin E Biosynthesis FEMS Microbiology Letters. May, 2013 | Pubmed ID: 23480614 Complete Genome Sequence of Saccharothrix Espanaensis DSM 44229(T) and Comparison to the Other Completely Sequenced Pseudonocardiaceae BMC Genomics. Sep, 2012 | Pubmed ID: 22958348 SnoaW/SnoaL2: a Different Two-component Monooxygenase Chemistry & Biology. May, 2012 | Pubmed ID: 22633406 Site-specific Recombination Strategies for Engineering Actinomycete Genomes Applied and Environmental Microbiology. Mar, 2012 | Pubmed ID: 22247163 SimReg1 is a Master Switch for Biosynthesis and Export of Simocyclinone D8 and Its Precursors AMB Express. Jan, 2012 | Pubmed ID: 22214346 Cloning and Heterologous Expression of Three Type II PKS Gene Clusters from Streptomyces Bottropensis Chembiochem : a European Journal of Chemical Biology. Jan, 2012 | Pubmed ID: 22162248 Genome Sequence of Streptomyces Sp. Strain Tü6071 Journal of Bacteriology. Aug, 2011 | Pubmed ID: 21705604 Rational Design of an Aryl-C-glycoside Catalyst from a Natural Product O-glycosyltransferase Chemistry & Biology. Apr, 2011 | Pubmed ID: 21513888 Chemoenzymatic and Bioenzymatic Synthesis of Carbohydrate Containing Natural Products Topics in Current Chemistry. 2010 | Pubmed ID: 21495258 Combinatorial and Synthetic Biosynthesis in Actinomycetes Fortschritte Der Chemie Organischer Naturstoffe = Progress in the Chemistry of Organic Natural Products. Progres Dans La Chimie Des Substances Organiques Naturelles. 2010 | Pubmed ID: 20556678 Functions of Genes and Enzymes Involved in Phenalinolactone Biosynthesis Chembiochem : a European Journal of Chemical Biology. Jul, 2010 | Pubmed ID: 20540056 Characterization and Analysis of the Regulatory Network Involved in Control of Lipomycin Biosynthesis in Streptomyces Aureofaciens Tü117 Applied Microbiology and Biotechnology. Jan, 2010 | Pubmed ID: 19585113 Production of Avilamycin A is Regulated by AviC1 and AviC2, Two Transcriptional Activators The Journal of Antibiotics. Aug, 2009 | Pubmed ID: 19609289 A Bacterial Glycosyltransferase Gene Toolbox: Generation and Applications Phytochemistry. Oct-Nov, 2009 | Pubmed ID: 19559449 Cloning and Sequencing of the Biosynthetic Gene Cluster for Saquayamycin Z and Galtamycin B and the Elucidation of the Assembly of Their Saccharide Chains Chembiochem : a European Journal of Chemical Biology. May, 2009 | Pubmed ID: 19384899 Chapter 12. The Power of Glycosyltransferases to Generate Bioactive Natural Compounds Methods in Enzymology. 2009 | Pubmed ID: 19374988 Differences in the Substrate Specificity of Glycosyltransferases Involved in Landomycins A and E Biosynthesis Applied Microbiology and Biotechnology. Jul, 2009 | Pubmed ID: 19352642 Genes and Enzymes Involved in Bacterial Isoprenoid Biosynthesis Current Opinion in Chemical Biology. Apr, 2009 | Pubmed ID: 19318289 Organisation of the Biosynthetic Gene Cluster and Tailoring Enzymes in the Biosynthesis of the Tetracyclic Quinone Glycoside Antibiotic Polyketomycin Chembiochem : a European Journal of Chemical Biology. Apr, 2009 | Pubmed ID: 19266534 Engineering a Function into a Glycosyltransferase Chemistry & Biology. Jan, 2009 | Pubmed ID: 19171303 Features and Applications of Bacterial Glycosyltransferases: Current State and Prospects Applied Microbiology and Biotechnology. Oct, 2008 | Pubmed ID: 18777021 Aranciamycin Analogs Generated by Combinatorial Biosynthesis Show Improved Antitumor Activity Applied Microbiology and Biotechnology. Aug, 2008 | Pubmed ID: 18553079 Marker Removal from Actinomycetes Genome Using Flp Recombinase Gene. Aug, 2008 | Pubmed ID: 18550297 Coordination of Export and Glycosylation of Landomycins in Streptomyces Cyanogenus S136 FEMS Microbiology Letters. Aug, 2008 | Pubmed ID: 18537830 Deoxysugars in Bioactive Natural Products: Development of Novel Derivatives by Altering the Sugar Pattern Current Topics in Medicinal Chemistry. 2008 | Pubmed ID: 18473893 Glycosyltransferases, Important Tools for Drug Design Current Topics in Medicinal Chemistry. 2008 | Pubmed ID: 18473892 Functional Expression of the Cre Recombinase in Actinomycetes Applied Microbiology and Biotechnology. Apr, 2008 | Pubmed ID: 18299828 Heterologous Expression and Genetic Engineering of the Phenalinolactone Biosynthetic Gene Cluster by Using Red/ET Recombineering Chembiochem : a European Journal of Chemical Biology. Feb, 2008 | Pubmed ID: 18157854 Function of LanI in Regulation of Landomycin A Biosynthesis in Streptomyces Cyanogenus S136 and Cross-complementation Studies with Streptomyces Antibiotic Regulatory Proteins Encoding Genes Archives of Microbiology. Feb, 2008 | Pubmed ID: 17786405 The Future of Natural Products As a Source of New Antibiotics Current Opinion in Investigational Drugs (London, England : 2000). Aug, 2007 | Pubmed ID: 17668363 Structure and Action of the C-C Bond-forming Glycosyltransferase UrdGT2 Involved in the Biosynthesis of the Antibiotic Urdamycin Journal of Molecular Biology. Sep, 2007 | Pubmed ID: 17640665 A Strategy for Cloning Glycosyltransferase Genes Involved in Natural Product Biosynthesis Applied Microbiology and Biotechnology. Jul, 2007 | Pubmed ID: 17497146 SorF: a Glycosyltransferase with Promiscuous Donor Substrate Specificity in Vitro Chembiochem : a European Journal of Chemical Biology. May, 2007 | Pubmed ID: 17407127 A Genomic Screening Approach to the Structure-guided Identification of Drug Candidates from Natural Sources Chembiochem : a European Journal of Chemical Biology. May, 2007 | Pubmed ID: 17407125 Cloning and Heterologous Expression of the Aranciamycin Biosynthetic Gene Cluster Revealed a New Flexible Glycosyltransferase Chembiochem : a European Journal of Chemical Biology. Apr, 2007 | Pubmed ID: 17357167 Type II Polyketide Synthases: Gaining a Deeper Insight into Enzymatic Teamwork Natural Product Reports. Feb, 2007 | Pubmed ID: 17268612 Generation of New Landomycins with Altered Saccharide Patterns Through Over-expression of the Glycosyltransferase Gene LanGT3 in the Biosynthetic Gene Cluster of Landomycin A in Streptomyces Cyanogenus S-136 Chembiochem : a European Journal of Chemical Biology. Jan, 2007 | Pubmed ID: 17139690 On the Acceptor Substrate of C-glycosyltransferase UrdGT2: Three Prejadomycin C-Glycosides from an Engineered Mutant of Streptomyces Globisporus 1912 DeltalndE(urdGT2) Angewandte Chemie (International Ed. in English). Nov, 2006 | Pubmed ID: 17061307 Biosynthesis of the Terpene Phenalinolactone in Streptomyces Sp. Tü6071: Analysis of the Gene Cluster and Generation of Derivatives Chemistry & Biology. Apr, 2006 | Pubmed ID: 16632249 Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix Espanaensis Journal of Bacteriology. Apr, 2006 | Pubmed ID: 16547054 The Active Conformation of Avilamycin A is Conferred by AviX12, a Radical AdoMet Enzyme The Journal of Biological Chemistry. May, 2006 | Pubmed ID: 16537546 A Putative Proteinase Gene is Involved in Regulation of Landomycin E Biosynthesis in Streptomyces Globisporus 1912 FEMS Microbiology Letters. Feb, 2006 | Pubmed ID: 16448507 Glycosyltransferases Involved in the Biosynthesis of Biologically Active Natural Products That Contain Oligosaccharides Molecular BioSystems. Jul, 2005 | Pubmed ID: 16880973 LanV, a Bifunctional Enzyme: Aromatase and Ketoreductase During Landomycin A Biosynthesis Chembiochem : a European Journal of Chemical Biology. Dec, 2005 | Pubmed ID: 16283688 Genes Encoding Enzymes Responsible for Biosynthesis of L-lyxose and Attachment of Eurekanate During Avilamycin Biosynthesis Chemistry & Biology. Oct, 2005 | Pubmed ID: 16242656 It Works: Combinatorial Biosynthesis for Generating Novel Glycosylated Compounds Molecular Microbiology. Oct, 2005 | Pubmed ID: 16164544 Iteratively Acting Glycosyltransferases Involved in the Hexasaccharide Biosynthesis of Landomycin A Chemistry & Biology. Jul, 2005 | Pubmed ID: 16039521 LanGT2 Catalyzes the First Glycosylation Step During Landomycin A Biosynthesis Chembiochem : a European Journal of Chemical Biology. Aug, 2005 | Pubmed ID: 15977274 Generation of Novel Landomycins M and O Through Targeted Gene Disruption Chembiochem : a European Journal of Chemical Biology. Apr, 2005 | Pubmed ID: 15812784 Exploiting Pseudomonas Putida for Drug Development Chemistry & Biology. Mar, 2005 | Pubmed ID: 15797207 Novel Natural Compounds Obtained by Genome-based Screening and Genetic Engineering Current Opinion in Drug Discovery & Development. Mar, 2005 | Pubmed ID: 15782546 Identification of the Function of Gene LndM2 Encoding a Bifunctional Oxygenase-reductase Involved in the Biosynthesis of the Antitumor Antibiotic Landomycin E by Streptomyces Globisporus 1912 Supports the Originally Assigned Structure for Landomycinone The Journal of Organic Chemistry. Jan, 2005 | Pubmed ID: 15651811 Genes Involved in Formation and Attachment of a Two-carbon Chain As a Component of Eurekanate, a Branched-chain Sugar Moiety of Avilamycin A Applied and Environmental Microbiology. Jan, 2005 | Pubmed ID: 15640214 Structure and Function of the Antibiotic Resistance-mediating Methyltransferase AviRb from Streptomyces Viridochromogenes Journal of Molecular Biology. Jan, 2005 | Pubmed ID: 15581897 Function of LanGT3, a Glycosyltransferase Gene Involved in Landomycin A Biosynthesis Chembiochem : a European Journal of Chemical Biology. Nov, 2004 | Pubmed ID: 15515090 Novel Avilamycin Derivatives with Improved Polarity Generated by Targeted Gene Disruption Chemistry & Biology. Oct, 2004 | Pubmed ID: 15489167 Generation of Streptomyces Globisporus SMY622 Strain with Increased Landomycin E Production and It's Initial Characterization The Journal of Antibiotics. Jun, 2004 | Pubmed ID: 15323127 The Glycosyltransferase UrdGT2 Catalyzes Both C- and O-glycosidic Sugar Transfers Angewandte Chemie (International Ed. in English). May, 2004 | Pubmed ID: 15170316 Rational Saccharide Extension by Using the Natural Product Glycosyltransferase LanGT4 Chembiochem : a European Journal of Chemical Biology. Mar, 2004 | Pubmed ID: 14997529 Expression of the Landomycin Biosynthetic Gene Cluster in a PKS Mutant of Streptomyces Fradiae is Dependent on the Coexpression of a Putative Transcriptional Activator Gene FEMS Microbiology Letters. Jan, 2004 | Pubmed ID: 14734170 The Avilamycin Resistance Determinants AviRa and AviRb Methylate 23S RRNA at the Guanosine 2535 Base and the Uridine 2479 Ribose Molecular Microbiology. Jul, 2003 | Pubmed ID: 12828631 Crystal Structure of the Avilamycin Resistance-conferring Methyltransferase AviRa from Streptomyces Viridochromogenes Journal of Molecular Biology. May, 2003 | Pubmed ID: 12742024 The C-Glycosyltransferase UrdGT2 is Unselective Toward D- and L-configured Nucleotide-bound Rhodinoses Journal of the American Chemical Society. Apr, 2003 | Pubmed ID: 12696864 Urdamycin L: a Novel Metabolic Shunt Product That Provides Evidence for the Role of the UrdM Gene in the Urdamycin A Biosynthetic Pathway of Streptomyces Fradiae TU 2717 Chembiochem : a European Journal of Chemical Biology. Jan, 2003 | Pubmed ID: 12512084 Rationally Designed Glycosylated Premithramycins: Hybrid Aromatic Polyketides Using Genes from Three Different Biosynthetic Pathways Journal of the American Chemical Society. May, 2002 | Pubmed ID: 12022840 Engineered Urdamycin Glycosyltransferases Are Broadened and Altered in Substrate Specificity Chemistry & Biology. Mar, 2002 | Pubmed ID: 11927254 From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028 Anja Greule1, Songya Zhang1, Thomas Paululat2, Andreas Bechthold1 1Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology, Universität Siegen JoVE 54952 Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028 Anja Greule1, Songya Zhang1, Thomas Paululat2, Andreas Bechthold1 1Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology, Universität Siegen JoVE 54952 Genetics