Articles by Angela D. Caldera-Siu in JoVE
Cirurgia estereotáxica sobrevivência em Roedores Brenda M. Geiger1, Lauren E. Frank1, Angela D. Caldera-Siu1, Emmanuel N. Pothos1 1Department of Pharmacology and Experimental Therapeutics, Tufts University O acompanhamento dos níveis de neurotransmissores extracelular em regiões distintas do cérebro de animais que circulam livremente oferece insights sobre a relação entre a liberação do neurotransmissor e comportamento. Em microdiálise in vivo juntamente com detecção eletroquímica fornece anatômicas excelentes e resolução de química; e informações sobre como a neurotransmissão basal é alterada por manipulações farmacológicas ou fisiológicas.
Principal Dissociated Midbrain dopamina culturas de células de recém-nascidos de roedores Lauren E. Frank1, Angela D. Caldera-Siu1, Emmanuel N. Pothos1 1Department of Pharmacology and Experimental Therapeutics, Tufts University Principal dissociada mesencéfalo culturas de células de dopamina permitir o estudo das características pré-sináptica dos neurônios dopaminérgicos. Eles podem ser usados para monitorar em tempo real a cinética de liberação de dopamina e proteína / mRNA níveis de reguladores de exocitose dopamina. Aqui, nós mostrar-lhe como gerar essas culturas de recém-nascidos de roedores.
Other articles by Angela D. Caldera-Siu on PubMed
Evidence for Defective Mesolimbic Dopamine Exocytosis in Obesity-prone Rats FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Aug, 2008 | Pubmed ID: 18477764 The association between dietary obesity and mesolimbic systems that regulate hedonic aspects of feeding is currently unresolved. In the present study, we examined differences in baseline and stimulated central dopamine levels in obesity-prone (OP) and obesity-resistant (OR) rats. OP rats were hyperphagic and showed a 20% weight gain over OR rats at wk 15 of age, when fed a standard chow diet. This phenotype was associated with a 50% reduction in basal extracellular dopamine, as measured by a microdialysis probe in the nucleus accumbens, a projection site of the mesolimbic dopamine system that has been implicated in food reward. Similar defects were also observed in younger animals (4 wk old). In electrophysiology studies, electrically evoked dopamine release in slice preparations was significantly attenuated in OP rats, not only in the nucleus accumbens but also in additional terminal sites of dopamine neurons such as the accumbens shell, dorsal striatum, and medial prefrontal cortex, suggesting that there may be a widespread dysfunction in mechanisms regulating dopamine release in this obesity model. Moreover, dopamine impairment in OP rats was apparent at birth and associated with changes in expression of several factors regulating dopamine synthesis and release: vesicular monoamine transporter-2, tyrosine hydroxylase, dopamine transporter, and dopamine receptor-2 short-form. Taken together, these results suggest that an attenuated central dopamine system would reduce the hedonic response associated with feeding and induce compensatory hyperphagia, leading to obesity.
Feeding Induced by Cannabinoids is Mediated Independently of the Melanocortin System PloS One. 2008 | Pubmed ID: 18493584 Cannabinoids, the active components of marijuana, stimulate appetite, and cannabinoid receptor-1 (CB1-R) antagonists suppress appetite and promote weight loss. Little is known about how CB1-R antagonists affect the central neurocircuitry, specifically the melanocortin system that regulates energy balance.