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Chapters
- 00:00Title
- 00:14Introduction
- 01:00Preparing Tools and Reagents
- 05:03Dissecting Midbrain of P0-P2 Rodent
- 09:10Triturating Brain Segments
- 10:49Plating Dissociated Neurons
- 12:53Primary Dissociated Midbrain Dopamine Cultures
- 13:28Conclusion
Principal dissociada mesencéfalo culturas de células de dopamina permitir o estudo das características pré-sináptica dos neurônios dopaminérgicos. Eles podem ser usados para monitorar em tempo real a cinética de liberação de dopamina e proteína / mRNA níveis de reguladores de exocitose dopamina. Aqui, nós mostrar-lhe como gerar essas culturas de recém-nascidos de roedores.
Tags
Primary Cell CulturesDopamine NeuronsPresynaptic CharacteristicsDopamine Release KineticsCarbon Fiber AmperometryDopamine Related GenesProteinsQuantitative PCRImmunocytochemistryRodent NeonatesCortical Glial AstrocytesConditioning Of Neuronal Cell Culture MediaMidbrain DissectionDigestionExtractionPlating Of Dopamine NeuronsNeurotrophic FactorsElectrophysiologyVideo MicroscopyCell Viability AssaysToxicological Screens
