In JoVE (1)
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Articles by Athar Ansari in JoVE
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach Zuzer Dhoondia1, Ricci Tarockoff1, Nadra Alhusini1, Scott Medler1, Neha Agarwal1, Athar Ansari1 1Department of Biological Sciences, Wayne State University We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.