In JoVE (1)
Articles by Austin Ferro in JoVE
Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function Austin Ferro1, Emily Carbone1, Evan Marzouk1, Asher Siegel2, Donna Nguyen1, Kailen Polley1, Jessilyn Hartman1, Kimberley Frederick2, Stephen Ives3, Sarita Lagalwar1 1Neuroscience Program, Skidmore College, 2Chemistry Department, Skidmore College, 3Health and Exercise Science Department, Skidmore College We present a biochemical and behavioral protocol to evaluate the efficacy of mitochondria-targeted water-soluble compounds for the treatment of Spinocerebellar ataxia type 1 (SCA1) and other cerebellar neurodegenerative diseases.
Other articles by Austin Ferro on PubMed
Lipidomic Profiling of Phosphocholine-containing Brain Lipids in Mice with Sensorimotor Deficits and Anxiety-like Features After Exposure to Gulf War Agents Neuromolecular Medicine. Dec, 2012 | Pubmed ID: 22798222 The central nervous system (CNS)-based symptoms of Gulf War Illness (GWI) include motor dysfunction, anxiety, and cognitive impairment. Gulf War (GW) agents, such as pyridostigmine bromide (PB), permethrin (PER), N,N-diethyl-meta-toluamide (DEET), and stress, are among the contributory factors to the pathobiology of GWI. This study characterizes disturbances in phosphocholine-containing lipids that accompany neurobehavioral and neuropathological features associated with GW agent exposure. Exposed mice received PB orally, dermal application of PER and DEET and restraint stress daily for 28 days, while controls received vehicle during this period. Neurobehavioral studies included the rotarod, open field, and Morris water maze tests. Histopathological assessments included glial fibrillary acid protein, CD45, and Nissl staining. Liquid chromatography/mass spectrometry with source collision-induced dissociation in negative and positive ionization scanning modes was performed to characterize brain phosphatidylcholine (PC) and sphingomyelin (SM). A significant increase in ether containing PC (ePC34:0, ePC36:2, and ePC36:1) or long-chain fatty acid-containing PC (38:1, 40:4, 40:2) was observed in exposed mice compared with controls. Among differentially expressed PCs, levels of those with monounsaturated fatty acids were more affected than those with saturated and polyunsaturated fatty acids. Sensorimotor deficits and anxiety, together with an increase in astrocytosis, were observed in exposed mice compared with controls. These lipid changes suggest that alterations in peroxisomal pathways and stearoyl-CoA desaturase activity accompany neurobehavioral and neuropathological changes after GW agent exposure and represent possible treatment targets for the CNS symptoms of GWI.
Chronic Neuropathological and Neurobehavioral Changes in a Repetitive Mild Traumatic Brain Injury Model Annals of Neurology. Feb, 2014 | Pubmed ID: 24243523 Traumatic brain injury (TBI) is a recognized risk factor for later development of neurodegenerative disease. However, the mechanisms contributing to neurodegeneration following TBI remain obscure.