In JoVE (1)
Other Publications (1)
Articles by Christopher P. Barkway in JoVE
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens Christopher P. Barkway1, Rebecca L. Pocock1, Vladimir Vrba2, Damer P. Blake1 1Department of Pathology and Pathogen Biology, Royal Veterinary College, London, 2BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
Other articles by Christopher P. Barkway on PubMed
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria That Infect Chickens BMC Veterinary Research. 2011 | Pubmed ID: 22053893 Eimeria parasites can cause the disease coccidiosis in poultry and even subclinical infection can incur economic loss. Diagnosis of infection predominantly relies on traditional techniques including lesion scoring and faecal microscopy despite the availability of sensitive molecular assays, largely due to cost and the requirement for specialist equipment. Despite longstanding proven efficacy these traditional techniques demand time and expertise, can be highly subjective and may under-diagnose subclinical disease. Recognition of the tight economic margins prevailing in modern poultry production and the impact of avian coccidiosis on poverty in many parts of the world has highlighted a requirement for a panel of straightforward and sensitive, but cost-effective, Eimeria species-specific diagnostic assays.