In JoVE (1)
Other Publications (0)
Articles by Dean A. Houston in JoVE
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification Dean A. Houston*1, Katherine A. Staines*1, Vicky E. MacRae1, Colin Farquharson1 1Developmental Biology, The Roslin Institute and R(D)SVS, The University of Edinburgh We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.