In JoVE (1)
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Articles by Emily S. Pyne in JoVE
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements Nabil E. Boutagy1,2, George W. Rogers3, Emily S. Pyne1, Mostafa M. Ali1, Matthew W. Hulver1,2, Madlyn I. Frisard1,2 1The Department of Human Nutrition, Foods, and Exercise, Virginia Tech, 2The Metabolic Phenotyping Core, Virginia Tech, 3Seahorse Bioscience The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.