Other Publications (1)
Articles by Jennifer Q. Kwong in JoVE
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells Joshua T. Maxwell1, Chin-Hsien Tsai1, Tahmina A. Mohiuddin2, Jennifer Q. Kwong1 1Department of Pediatrics, Emory University School of Medicine, 2Emory College of Arts and Sciences, Emory University Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.
Other articles by Jennifer Q. Kwong on PubMed
The Mitochondrial Calcium Uniporter in the Heart: Energetics and Beyond The Journal of Physiology. | Pubmed ID: 27991671 Ca and mitochondria are inextricably linked to cardiac function and dysfunction. Ca is central to cardiac excitation-contraction coupling and stimulates mitochondrial energy production to fuel contraction. Under pathological conditions of dysregulated Ca cycling, mitochondrial Ca overload activates cellular death pathways. Thus, in the cardiomyocyte, the mitochondrial Ca microdomain is where contraction, energy and death collide. A key component of mitochondrial Ca signalling is the mitochondrial Ca uniporter complex (uniplex), an inner membrane Ca transporter and major pathway of mitochondrial Ca entry. Once known only as the unidentified target for ruthenium red and related compounds, in recent years, the uniplex has evolved into a complex multiprotein assembly. The identification of the molecular constituents of the uniplex has made possible the generation of targeted genetic models to interrogate uniplex function in vivo. This review will summarize our current understanding of the molecular structure of the uniplex, its impact on mitochondrial energetics and cardiac physiology, its contribution to cardiomyocyte death, and its expanding roles in cardiac biology.