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Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
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Biochemistry
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JoVE Journal Biochemistry
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

DOI:
10.3791/57225-v

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08:29 min

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April 27, 2018

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, , ,
1Department of Pediatrics,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

Chapters

  • 00:04Title
  • 00:56Mitochondrial Calcium Uptake Measurement in Isolated Cardiac Mitochondria Using a Plate Reader
  • 03:33Mitochondrial Calcium Measurement Using Confocal Imaging in Cultured Cells
  • 06:14Results: Mitochondrial Calcium Analysis in Isolated Mitochondria and in Cultured Cells
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

Tags

Keywords: Mitochondrial Calcium InfluxCalcium SignalingMitochondrial Calcium DynamicsDisease ConditionsPlate-reader-based Mitochondrial Calcium UptakeConfocal-microscope-based Mitochondrial Calcium InfluxIsolated HeartMSEGTA BufferCalcium Green-5NCalcium Chloride

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