Other Publications (1)
Articles by Jitka Y. Sagiv in JoVE
Isolation and Characterization of Neutrophils with Anti-Tumor Properties Ronit Vogt Sionov1, Simaan Assi1, Maya Gershkovitz1, Jitka Y. Sagiv1, Lola Polyansky1, Inbal Mishalian2, Zvi G. Fridlender2, Zvi Granot1 1Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 2Institute of Pulmonary Medicine, Hadassah-Hebrew University Medical Center Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.
Other articles by Jitka Y. Sagiv on PubMed
Phenotypic Diversity and Plasticity in Circulating Neutrophil Subpopulations in Cancer Cell Reports. Feb, 2015 | Pubmed ID: 25620698 Controversy surrounds neutrophil function in cancer because neutrophils were shown to provide both pro- and antitumor functions. We identified a heterogeneous subset of low-density neutrophils (LDNs) that appear transiently in self-resolving inflammation but accumulate continuously with cancer progression. LDNs display impaired neutrophil function and immunosuppressive properties, characteristics that are in stark contrast to those of mature, high-density neutrophils (HDNs). LDNs consist of both immature myeloid-derived suppressor cells (MDSCs) and mature cells that are derived from HDNs in a TGF-β-dependent mechanism. Our findings identify three distinct populations of circulating neutrophils and challenge the concept that mature neutrophils have limited plasticity. Furthermore, our findings provide a mechanistic explanation to mitigate the controversy surrounding neutrophil function in cancer.