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Isolation and Characterization of Neutrophils with Anti-Tumor Properties
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

DOI:
10.3791/52933-v

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10:15 min

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June 19, 2015

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, , , , , , ,
1Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada,Hebrew University Medical School, 2Institute of Pulmonary Medicine,Hadassah-Hebrew University Medical Center

Chapters

  • 00:05Title
  • 01:15Cytotoxic Neutrophil Isolation from the Blood of Tumor-bearing Mice
  • 03:43Circulating Neutrophil Isolation from Cancer Patients
  • 05:07Thioglycollate- or Zymosan-elicited Neutrophil Isolation
  • 06:08Cytological Staining of Isolated Neutrophils
  • 06:48In vitro Luciferase Analysis of Isolated Neutrophil Anti-tumor Activity
  • 08:15Results: Representative Neutrophil Functionality
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.

Tags

Keywords: NeutrophilsAnti-tumor PropertiesTumor-entrained Neutrophils (TEN)Low-density Neutrophils (LDN)IsolationCharacterizationAnti-tumor ActivityMigrationReactive Oxygen SpeciesPhagocytosisIn Vivo TrackingAnti-metastatic Capacity

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