Laura Miesen

Department of Pathology.

Radboud university medical center, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands

Laura Miesen
MSc

My name is Laura Miesen. I am a PhD candidate at the department of Pathology, Radboudumc. I am part of the Nephropathology group which is lead by Dr. ing. Bart Smeets. The main aim of my PhD is to unravel the molecular mechanisms leading to the activation of parietal epithelial cells (PECs) in the kidney. Activated PECs are responsible for scar tissue formation in the glomeruli, the filtration units of our kidneys. Inhibiting PEC activation will possibly lead to the reduction of renal failure (end stage renal disease). The laboratory techniques I use are: Immunostainings (IHC, IF), RNAscope, cell culture, cell assays, mice experiments, microscopy, PCR, RT-PCR, qPCR, western blot, scRNAseq (in collaboration).

Publications

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

1Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center

JoVE 60324

 Biology