In JoVE (8)
- High-Throughput, Multi-Image Cryohistology of Mineralized Tissues
- Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
- An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration
- Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
- A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
- Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult
- Stress Distribution During Cold Compression of Rocks and Mineral Aggregates Using Synchrotron-based X-Ray Diffraction
- Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
Other Publications (0)
Articles by Li Chen in JoVE
High-Throughput, Multi-Image Cryohistology of Mineralized Tissues Nathaniel A. Dyment1, Xi Jiang1, Li Chen1, Seung-Hyun Hong2, Douglas J. Adams3, Cheryl Ackert-Bicknell4, Dong-Guk Shin2, David W. Rowe1 1Department of Reconstructive Sciences, University of Connecticut Health Center, 2Department of Computer Science and Engineering, University of Connecticut, 3Department of Orthopaedic Surgery, University of Connecticut Health Center, 4Department of Orthopaedics, University of Rochester In this manuscript, we present a high-throughput, semi-automated cryohistology platform to produce aligned composite images of multiple response measures from several rounds of fluorescent imaging on frozen sections of mineralized tissues.
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass Hieng Chiong Tie1, Bing Chen1, Xiuping Sun1, Li Cheng2,3, Lei Lu1 1School of Biological Sciences, Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing, National University of Singapore The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.
An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration Wenze Cao*1,2,3, Li Song*1,2,3, Jingjing Cheng1,2,3, Na Yi1,2,3, Luyi Cai1,2,3, Fu-de Huang4,5, Margaret Ho1,2,3 1Research Center for Translational Medicine, Tongji University School of Medicine, 2Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, 3Department of Anatomy and Neurobiology, Tongji University School of Medicine, 4Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 5Sino-Danish College, University of Chinese Academy of Sciences, Chinese Academy of Sciences This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices Yangjian Feng*1,2, Binliang Tang*1,2, Ming Chen2, Li Yang1,3,4,5 1School of Psychology, South China Normal University, 2School of Life Sciences, South China Normal University, 3Brain Science Institute, South China Normal University, 4Center for Studies of Psychological Application, South China Normal University, 5Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites Li Xie1, Liqin Chen3, Pan Gu4, Lanlan Wei1, Xuejun Kang2 1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.
Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult Stephanie A. Herrlinger1, Qiang Shao2, Li Ma2, Melinda Brindley3, Jian-Fu Chen2 1Biomedical and Health Sciences Institute, University of Georgia, 2Center for Craniofacial Molecular Biology, University of Southern California, 3Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia Here we describe a method for establishing a model of Zika virus-induced microcephaly in mouse. This protocol includes methods for embryonic, neonatal, and adult-stage intracerebral inoculation of the Zika virus.
Stress Distribution During Cold Compression of Rocks and Mineral Aggregates Using Synchrotron-based X-Ray Diffraction Cecilia S.N. Cheung1,2, Donald J. Weidner1, Li Li1, Philip G. Meredith3, Haiyan Chen1, Matthew Whitaker1, Xianyin Chen4 1Mineral Physics Institute, Department of Geoscience, Stony Brook University, 2Geological Engineering, Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 3Rock and Ice Physics Laboratory, Department of Earth Sciences, University College London, 4Department of Chemistry, Stony Brook University We report detailed procedures for compression experiments on rocks and mineral aggregates within a multi-anvil deformation apparatus coupled with synchrotron X-radiation. Such experiments allow quantification of the stress distribution within samples, that ultimately sheds light on compaction processes in geomaterials.
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array Yasumasa Mashimo1,2, Momoko Yoshioka1, Yumie Tokunaga1, Christopher Fockenberg1, Shiho Terada1, Yoshie Koyama1, Teiko Shibata-Seki2, Koki Yoshimoto1, Risako Sakai1, Hayase Hakariya1, Li Liu1, Toshihiro Akaike3, Eiry Kobatake2, Siew-Eng How4, Motonari Uesugi1,5, Yong Chen1,6, Ken-ichiro Kamei1 1Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 5Institute for Chemical Research, Kyoto University, 6Ecole Normale Supérieure This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.