Articles by Lucy Thorne in JoVE
Identification of Protein Interacting Partners Using Tandem Affinity Purification Dalan Bailey*1, Luis Urena*1, Lucy Thorne1, Ian Goodfellow1 1Section of Virology, Department of Medicine, Imperial College London Tandem affinity purification is a robust approach for the identification of protein binding partners. As proof of concept, this methodology was applied to the well-characterized translation initiation factor eIF4E to co-precipitate the host cell factors involved in translation initiation. This method is easily adapted to any cellular or viral protein.
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus Armando Arias*1, Luis Ureña*1, Lucy Thorne1, Muhammad A. Yunus1, Ian Goodfellow1 1Section of Virology, Imperial College London Noroviruses are a major cause of gastroenteritis yet molecular techniques for their characterisation are still relatively new. Here we report two different reverse genetics approaches for the efficient recovery of murine norovirus (MNV), the only member of this genus which can be propagated in cell culture.
Other articles by Lucy Thorne on PubMed
Interleukin 18 Coexpression During Respiratory Syncytial Virus Infection Results in Enhanced Disease Mediated by Natural Killer Cells Journal of Virology. Apr, 2010 | Pubmed ID: 20130064 Respiratory syncytial virus (RSV) causes bronchiolitis, the main cause of infantile hospitalization. Immunity against reinfection is poor, and there is great interest in boosting vaccine responses using live vectors expressing host cytokines. We therefore constructed a recombinant RSV expressing murine interleukin 18 (RSV/IL-18), a cytokine capable of inducing strong antiviral immune responses. In vitro RSV/IL-18 replicated at wild-type levels and produced soluble IL-18. In naÃ¯ve BALB/c mice, RSV/IL-18 infection significantly increased both IL-18 mRNA and protein and attenuated the peak viral load 3-fold. Despite a reduced viral load, RSV/IL-18 infection caused a biphasic weight loss at days 2 and 6 postinfection that was not seen in wild-type infection. Day 2 disease was associated with enhanced pulmonary natural killer (NK) cell numbers and activity and was prevented by NK cell depletion during infection; day 6 disease was correlated with CD8 T-cell recruitment and was enhanced by NK cell depletion. IL-18 expression during priming also enhanced RSV-specific antibody responses and T-cell responses on secondary RSV infection. Therefore, while IL-18 boosted antiviral immunity and reduced the viral load, its coexpression worsened disease. This is the first recombinant RSV with this property, and these are the first studies to demonstrate that NK cells can induce pathology during pulmonary viral infections.