In JoVE (1)
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Articles by Ludovic Collin in JoVE
High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis Roberto Villaseñor, Ludovic Collin 1Roche Pharma Research and Early Development (pRED), Neuroimmunology, Roche Innovation Center Basel Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.