In JoVE (1)
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Articles by M. Helen Huls in JoVE
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System Pappanaicken Kumaresan*1, Mathew Figliola*1, Judy S. Moyes1, M. Helen Huls1, Priti Tewari1, Elizabeth J. Shpall2, Richard Champlin2, Laurence J.N. Cooper1 1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.